Non‐Clinical
Aramchol™ significantly down regulates the three key pathologies of NASH: Steatosis, Inflammation and Fibrosis in multiple animal models.
Aramchol™ significantly reduces liver steatosis & inflammation in MCD diet model▷ Methionine and Choline Deficient (MCD) diet induces aminotransferase elevation and changes in hepatic histological features characterized by steatosis, local inflammation, hepatocyte necrosis and fibrosis.
▷ The MCD is deficient in two essential factors for the synthesis of phosphatidylcholine, which is involved in VLDL production and secretion from the liver.
▷ The MCD is one of the most commonly used animal models of NASH, as it induces a liver pathology that resembles the sequence and progression of liver pathology seen in humans.
Delire, B., Stärkel, P., & Leclercq, I. (2015). Animal models for fibrotic liver diseases: what we have, what we need, and what is under development. Journal of Clinical and Translational Hepatology, 3(1), 53.
▷ The MCD is deficient in two essential factors for the synthesis of phosphatidylcholine, which is involved in VLDL production and secretion from the liver.
▷ The MCD is one of the most commonly used animal models of NASH, as it induces a liver pathology that resembles the sequence and progression of liver pathology seen in humans.
Delire, B., Stärkel, P., & Leclercq, I. (2015). Animal models for fibrotic liver diseases: what we have, what we need, and what is under development. Journal of Clinical and Translational Hepatology, 3(1), 53.
Iruarrizaga‐Lejarreta, Marta, et al. “Role of Aramchol in steatohepatitis and fibrosis in mice.” Hepatology Communications 1.9 (2017): 911–927.
Aramchol™ significantly down regulates/normalized liver fibrosis in MCD diet model
Iruarrizaga‐Lejarreta, Marta, et al. “Role of Aramchol in steatohepatitis and fibrosis in mice.” Hepatology Communications 1.9 (2017): 911–927.
Aramchol™ significantly reduces liver fibrosis in TAA model▷ Thioacetamide (TAA) is the most commonly used toxic agent to induce liver fibrosis.
▷ Repeated IP injections of TAA leads to severe fibrosis /cirrhosis.
▷ Of all models for fibrosis, the TAA model shares multiple characteristics with human liver fibrosis and is considered to best predict efficacy in humans.
Delire, B., Stärkel, P., & Leclercq, I. (2015). Animal models for fibrotic liver diseases: what we have, what we need, and what is under development. Journal of Clinical and Translational Hepatology, 3(1), 53.
▷ Repeated IP injections of TAA leads to severe fibrosis /cirrhosis.
▷ Of all models for fibrosis, the TAA model shares multiple characteristics with human liver fibrosis and is considered to best predict efficacy in humans.
Delire, B., Stärkel, P., & Leclercq, I. (2015). Animal models for fibrotic liver diseases: what we have, what we need, and what is under development. Journal of Clinical and Translational Hepatology, 3(1), 53.
R. Golan‐Gerstl1, M. Valitsky1, R. Oren1, E. Brazovski2, L. Hayardeny1, S. Shimon Reif. “The anti‐fibrotic effect of aramchol on liver fibrosis in TAA animal model” (2017); The international liver congress (EASL), Amsterdam, the Netherlands.
Aramchol™ down regulates stellate cell proliferation and collagen production▷ The human hepatic cell line LX‐2 has been described as tool to study mechanisms of hepatic fibrogenesis and the testing of antifibrotic compounds.
▷ The human HSC cell lines Lieming Xu‐1 (LX‐1) and Lieming Xu‐2 (LX‐2) were originally generated by transformation of cultured primary HSC obtained from a male human liver with a plasmid encoding the SV40 large T‐antigen expressed under the control of a Rous sarcoma virus promoter (LX‐1) or by spontaneous immortalization of a subset of early passaged LX‐1 cells that were grown in low serum conditions (LX‐2).
Weiskirchen, Ralf, et al. “Genetic characteristics of the human hepatic stellate cell line LX‐2.” PloS one 8.10 (2013): e75692
▷ The human HSC cell lines Lieming Xu‐1 (LX‐1) and Lieming Xu‐2 (LX‐2) were originally generated by transformation of cultured primary HSC obtained from a male human liver with a plasmid encoding the SV40 large T‐antigen expressed under the control of a Rous sarcoma virus promoter (LX‐1) or by spontaneous immortalization of a subset of early passaged LX‐1 cells that were grown in low serum conditions (LX‐2).
Weiskirchen, Ralf, et al. “Genetic characteristics of the human hepatic stellate cell line LX‐2.” PloS one 8.10 (2013): e75692
Iruarrizaga‐Lejarreta, Marta, et al. “Role of aramchol in steatohepatitis and fibrosis in mice. “Hepatology Communications 1.9 (2017): 911–927.
